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Figure. Evolutionary relationships of Gloiodon (Minimum Evolution-Tree)

The evolutionary history was inferred using the Minimum Evolution method. The optimal tree with the sum of branch length = 0.30938192 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Maximum Composite Likelihood method and are in the units of the number of base substitutions per site. The ME tree was searched using the Close-Neighbor-Interchange (CNI) algorithm at a search level of 2. The Neighbor-joining algorithm was used to generate the initial tree. The analysis involved 21 nucleotide sequences. All positions with less than 95% site coverage were eliminated. That is, fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position. There were a total of 878 positions in the final dataset. Phylogenetic and molecular evolutionary analyses were conducted using MEGA version 5 (Tamura, Peterson, Peterson, Stecher, Nei, and Kumar 2011). All sequences were obtained from the Genbank and were aligned by the Muscle algorithm with standard settings.
List of the GenBank Sequences.

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